1. Are you using synthesis for R&D or production? Different laboratory settings require varying levels of
regulation. Typically, production facilities require specific
regulatory documents and services to ensure quality and
consistency. Some companies selling DNA RNA oligonucleotide
synthesizer only offer instruments and do not offer the necessary
documents and services required for production facilities. Services
and documents may include Instrument Qualification (IQ),
Operational Qualification (OQ), Preventative Maintenance (PM) and
more.
2. Are you looking for a turnkey, all-in-one oligonucleotide
synthesis solution? Deciding on a DNA RNA oligonucleotide synthesis is only a part of
the entire oligonucleotide synthesis process that needs to be
considered. Synthesis consumable compatibility and synthesis
protocols are examples of other decisions that play a role in the
consideration of purchasing an oligonucleotide synthesizer. Does
the synthesizer come with working protocols to get oligonucleotide
synthesis experiments started? Are the protocols compatible to work
with readily available synthesis consumables? Are synthesis
protocols easily modifiable using the instrument’s software?
3. Are you using any special modifications such as dyes, spacers,
or non-standard amidites? Oligonucleotide synthesizers can vary in reagent configuration.
Depending on the synthesizer, number of bottle reagents and tubing
can vary. Determining the number of special reagents you will need
will determine the number of reagent bottle positions you need.
Another feature you need to consider is the instrument’s software
capabilities. Is the instrument able to easily distinguish and
dispense from special reagents? Are the coupling times and other
parameters modifiable? How does the instrument handle special
modifications to the oligo?
4. On average, how many oligos do you plan to make per day or week
or month or year? Determining the amount of oligos you plan on synthesizing will
help determine your oligo throughput, or rate at which you will be
synthesizing oligos. DNA RNA Oligonucleotide synthesizers can range
from low / medium throughput, to high / ultra-high throughput.
Depending on your use case, a small to medium throughput oligo
synthesizer is a great choice for smaller laboratories looking to
experiment with different molecules, or just need a few oligos per
day / week. A high / ultra-high throughput instrument may be better
suited for larger production ready laboratories, or any laboratory
that needs higher yields.
5.How many oligos can your instruments make in a day? The number of oligos that an instrument can make per day depends
on the length of your oligo. Honya synthesizers are offered in
medium, high, and ultra-high throughput to match any output your
project may need.
6.What is maintenance like? HonyaBio offers varying levels of service plans, preventative
maintenance, instrument quality, and operational quality services
to keep your instrument running smoothly. Let us handle the
maintenance so you can focus on your experiments.
7. Which instrument do you recommend? We offer a variety of instruments and accessories for every
project. Our knowledgeable engineers and sales team will happily
guide you through your purchasing journey, and to find the right
instrument for your use case.
